![]() Within a few centimeters of free-space propagation, the illumination light picks up partial spatial coherence, the extent of which is sufficient to record lensfree in-line holograms of the objects flowing within the micro-fluidic channel. In this approach, termed Holographic Opto-fluidic Microscopy ( HOM), a spatially incoherent or partially coherent visible light source (quasi-monochromatic with a spectral bandwidth of 5-10 nm) illuminates the objects within a micro-fluidic channel as depicted in Fig. In general, microfluidics enabled on-chip digital microscopy could especially be important for global health problems to assist diagnosis of disease (e.g., malaria or tuberculosis) in remote locations, and holds significant promise not only for point-of-care operation but also for telemedicine applications if a sufficiently high spatial resolution and throughput can be achieved on the same platform.įor this purpose, here we demonstrate a new method to conduct lensfree opto-fluidic microscopy, which relies on partially coherent digital in-line holography and multi-frame Pixel Super-Resolution to create high-resolution on-chip images of the objects that are flowing within a micro-fluidic channel. Opto-Fluidic Microscopy (OFM) is another concept that came out of this emerging field, which aims to image objects flowing within a micro-fluidic channel without the use of any lenses. Opto-fluidic technologies would further enhance the performance and functionality of existing lab-on-a-chip platforms, and toward this end various opto-fluidic devices have been demonstrated including tunable lasers, lenses, waveguides and sensors. The cost-effectiveness and compactness of lab-on-a-chip devices when combined with throughput and sensitivity have already enabled powerful solutions to a wide range of biomedical problems. Opto-fluidics is an emerging field that aims to merge the available toolset of optics and microfluidics to create more flexible and reconfigurable optical devices with novel functionalities that can be incorporated into lab-on-a-chip platforms. HOM does not involve complicated fabrication processes or precise alignment, nor does it require a highly uniform flow of objects within microfluidic channels. ![]() elegans, Giardia lamblia, and Mulberry pollen. Our imaging modality utilizes partially coherent in-line holography and pixel super-resolution to create high-resolution amplitude and phase images of the objects flowing within micro-fluidic channels, which we demonstrate by imaging C. This imaging modality complements the miniaturization provided by microfluidics and would allow the integration of microscopy into existing on-chip microfluidic devices with various functionalities. Here we present a new opto-fluidic microscopy modality, i.e., Holographic Opto-fluidic Microscopy (HOM), based on lensless holographic imaging. ![]() ![]() ![]() Microfluidics has also expanded into optics to create reconfigurable and flexible optical devices such as reconfigurable lenses, lasers, waveguides, switches, and on-chip microscopes. Over the last decade microfluidics has created a versatile platform that has significantly advanced the ways in which micro-scale organisms and objects are controlled, processed and investigated, by improving the cost, compactness and throughput aspects of analysis. ![]()
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